复制因子C亚基3基因突变对拟南芥叶片生长发育的影响

摘  要： 在EMS诱变的突变体筛选中，获得了一个复制因子C亚基3编码基因的点突变体，被命名为rfc3-1。与复制因子C亚基1编码基因突变体rfc1-1、rfc1-2 和 rfc1-3不同，rfc3-1叶型狭长，生长发育受到显著的影响。本文以rfc3-1突变体纯合子植株和野生型拟南芥植株为材料，研究RFC3对拟南芥叶片生长发育的影响。
关键词：拟南芥；RFC3；叶片；生长发育

Effects of RFC3 Mutation on Leaf Growth and Development in Arabidopsis
Abstract：In a mutant screening of EMS mutagenesis, a point mutant of RFC3 was obtained and thus it was named rfc3-1. With dwarfed and have smaller and narrower leaves, the growth and development of rfc3-1 plants is affected significantly which is very different from mutant rfc1-1, rfc1-2 and rfc1-3. In the paper, rfc3-1 homozygotes and wild-type plants were used as materials to study the effects of RFC3 mutation on leaf growth and development in Arabidopsis thaliana.  

Key words：Arabidopsis thaliana; RFC3; leaf; growth and development

目    录    8000字
摘  要    1
关键词    1
1 前言    1
1.1拟南芥简述    1
1.2 复制因子C亚基3基因的介绍    2
2 材料与方法    4
2.1  材料    4
2.1.1 植物材料    4
2.1.2 实验仪器及试剂    4
2.2 试验方法    4
2.2.1 拟南芥的预处理    4
2.2.2 拟南芥的种植    5
2.2.3 拟南芥的移盆    5
2.2.4 拟南芥的观察    5
2.2.5 拟南芥叶片的石蜡切片制作    5
2.2.6 徒手切片    7
3  结果与分析    8
3.1野生型和突变型植株的生长情况对照    8
3.2复制因子C亚基３基因突变对拟南芥莲座状真叶数目的影响    8
3.3复制因子C亚基３基因突变对拟南芥真叶的影响    8
3.4 突变型植株和野生型植株真叶叶肉细胞对照    9
4  讨论    10
4.1 RFC3在细胞复制和增殖中的作用    10
4.2 材料的选取    10
4.3 拟南芥的种植    10
参考文献    11
致    谢    13