灰绿曲霉突变株的选育

灰绿曲霉突变株的选育(含选题审批表,任务书,开题报告,中期检查表,毕业论文9800字)
摘  要：本试验以黑砖茶为原料，从中分离出灰绿色真菌，通过对其菌落形态观察，和对其孢子进行显微观察以及电镜观察，并通过PCR反应扩增其ITS序列进行比对，以此为基础建立系统发育树，因而从细胞和分子水平上鉴定提取出的灰绿色真菌为灰绿曲霉。将获得的原始灰绿曲霉进行紫外诱变，对比诱变后的菌株与原始菌株在菌落形态和细胞形态上都有差异。两者ITS序列中第67号碱基、255号碱基、387号碱基、388号碱基、521号碱基五处碱基不同，其他碱基序列都相同，以此证明成功的筛选出了灰绿曲霉的突变株。
关键词：灰绿曲霉；ITS序列；分子鉴定；PCR扩增；诱变

Screening of Mutants of Aspergilling Glaucus
Abstract: Based on raw materials of black brick tea, the experiment aimed at identifying Aspergillus glaucus isolated at the cellular and molecular level by building a phylogenetic tree basing on observation on colony morphosis, microscopic and sub-microscopic observation on spores in combination with amplifying corresponding ITS sequence via PCR. There has been distinction in both colonial and cellular morphosis between the UV-irratdiated strain and the original strain isolated. ITS sequence of both the strains proved to be fully homologous except 67bp, 255bp, 387bp, 388bp and 521bp, which underlying the conclusion that a mutant of Aspergillus glaucus has been sucssesfully isolated after screening .
Key words: Aspergillus glaucus; ITS sequence; Molecular identifiction; PCR  mutagenesis
 

目  录  
摘要……………………………………………………………………………………1
关键词…………………………………………………………………………………1
1前言…………………………………………………………………………………1
1.1    茶……………………………………………………………………………1
1.2灰绿曲霉高产纤维素酶突变株的选育研究现状…………………………2
1.3研究手段……………………………………………………………………3
1.3.1形态学………………………………………………………………3
1.3.2分子生物学…………………………………………………………4
1.3.3其他…………………………………………………………………4
    1.4微生物在茶叶中的作用……………………………………………………5
2材料与方法…………………………………………………………………………6
    2.1材料 ………………………………………………………………………6
        2.1.1试验原材料…………………………………………………………6
        2.1.2实验试剂……………………………………………………………6
2.1.3主要仪器……………………………………………………………6
2.2 方法…………………………………………………………………………7
2.2.1 灰绿曲霉的分离纯化………………………………………………7
2.2.2 分离菌株的初步鉴定………………………………………………7
2.2.3 分离菌株的分子鉴定………………………………………………7     
2.2.4 分离菌株的诱变处理………………………………………………9
2.2.5诱变菌株DNA的提取，ITS序列扩增…………………………9
3结果与分析…………………………………………………………………………9
3.1试验结果……………………………………………………………………9
3.2分析讨论…………………………………………………………………15
4结论……………………………………………………………………………… 16
参考文献 ………………………………………………………………………16
致谢……………………………………………………………………………17