蜘蛛毒素表达序列标签分析

蜘蛛毒素表达序列标签分析(含选任务书,开题报告,中期检查表,毕业论文9100字)
摘  要：基因表达序列标签（Expressed Sequence Tag,EST）是从cDNA文库中随机挑取单克隆进行测序，所获得的序列片段，序列长度一般约为50-500bp。EST技术是功能基因组研究的重要研究手段。本文在白额高脚蛛毒素cDNA文库挑选并测序20个表达序列标签，通过EBI（欧洲生物信息学中心European Bioinformatics Institute），NCBI（美国国立生物技术信息中心 The National Center for Biotechnology Information），ExPasy(瑞士生物信息研究所下属的蛋白质分析专家系统 Expert Protein Analysis System)网页，利用其提供的软件工具如：BLASTX，BLASTN，Translate，SignalP，ClustalW2等，进行生物信息学分析。根据基因表达谱，可系统、全面地从mRNA水平了解特定细胞、组织或器官的基因表达模式，并描述和解释其生理功能。
关键词：蜘蛛毒素；毒素基因；cDNA文库；表达序列标签

Analysis of Expressed Sequence Tags of Spider Toxin
Abstract：The obtained gene expressed sequence tags (Expressed Sequence Tag, EST) are randomly picked from cDNA library by monoclonal sequencing. The sequence length is about 50-500bp.EST is the vital research method on functional genomics research. In this paper, the amount of 20 ESTs form Heteropoda venatoria cDNA library was sequenced. The tools were used to analysis the ESTs which are including BLASTX, BLASTN, Translate, SingalP, ClustalW2 from the EBI (European Bioinformatics Centre, European Bioinformatics Institute), NCBI (National Center for Biotechnology Information The National Center for Biotechnology Information) and ExPasy (subsidiary of the Swiss Institute of Bioinformatics Expert Protein Analysis System Expert Protein Analysis System) website. The gene expression models of specific cell tissue or organ can be understood systematically and comprehensively in mRNA level.
Key words: Spider Toxin; Toxin genes; cDNA Library; Expressed sequence tags
 

目    录
摘  要    1
关键词    1
1  前言    2
1.1  EBI    4
1.2  NCBI    5
1.2.1  基本研究    6
1.2.2  数据库和软件    6
1.3  ExPasy    7
1.3.1  ExPASy Proteomics tools    7
1.3.2  PROSITE database    7
1.3.3  SWISS-2DPAGE    8
1.3.4  SWISS-MODEL    8
1.3.5  SWISS-PROT    8
2  实验任务和工具    8
2.1  主要任务    8
2.2  实验工具    9
3  实验材料及方法    9
3.1  材料    9
3.2  方法    9
4  操作及分析    11
4.1  菌落PCR结果    11
4.2  翻译    11
4.3  同源性比对分析    13
4.4  信号肽酶切位点确定    16
4.5  聚类分析    20
5  实验总结    25
参考文献    25
致    谢    27