致病力减弱的稻瘟菌转化子筛选

致病力减弱的稻瘟菌转化子筛选(8800字)
摘 要：活化菌株接种大麦通过对照比对筛选出致病力减弱的菌株。本研究通过活化稻瘟P131小种ATMT（Agrobacterium-madiated transformation）并产孢培养，待大麦在一叶两星状态时接种。观察发病叶片上的病斑类型，数量和大小，通过与p131野生菌对照，筛选出发病程度明显减弱的叶片，也就是致病力减弱的转化子，再进行复筛确认。本研究通过筛选稻瘟P131小种ATMT（Agrobacterium-madiated transformation）转化体库中的获得2个致病力不同程度减弱的转化体SX6140、SX6505。

关键字：稻瘟菌；ATMT；致病力；接种

SCREENING OF THE REDUCED VIRULENCE OF MAGNAPORTHE GRISEA TRANSFORMANTS
Abstract: Activation strains were inoculated by contrast ratio of barley weakening virulence strain. This study through the activation ATMT P131 small kind of rice blast Agrobacterium - madiated transformation (and spore production is to foster, barley in state two stars vaccinations. Observe the disease spot on the leaf of type, quantity and size, through contrast with p131 wild, and screened the degree of disease weakened obviously leaves, is also weakened virulence, again carries on the transformation of complex son screen confirmation.This study by screening for rice blast of Agrobacterium P131 small ATMT (madiated transformation) transformation body - repository.By 2 irulence of different extent SX6140, weakened SX6505 into body.

Key word：Magnaporthe grisea；ATMT；pathogenicity；inoculate
 

目    录
摘要 ………………………………………………………………………………………………4
关键词 ……………………………………………………………………………………………4
1.1前言 ………………………………………………………………………………………… 5
1.2稻瘟菌的生物学特性…………………………………………………………………………5
1.2.1稻瘟菌的生活史 …………………………………………………………………… 5
1.2.2稻瘟菌的侵染过程 ………………………………………………………………… 6
1.3稻瘟菌是研究植物病原真菌的模式菌 …………………………………………………… 7
1.4稻瘟菌ＡＴＭＴ转化子库的构建…………………………………………………………… 7
1.5  研究目的 ………………………………………………………………………………… 8
2 材料与方法…………………………………………………………………………………… 8  
2. 1 供试材料………………………………………………………………………………… 8
2. 2 实验方法………………………………………………………………………………… 9
    2. 2. 1 培养基的选择与制作…………………………………………………………… 9
    2. 2. 2 菌种的活化……………………………………………………………………… 9
    2. 2. 3 产孢………………………………………………………………………………10
        2. 2. 3. 1 涂菌………………………………………………………………………10
        2. 2. 3. 2 洗菌………………………………………………………………………10
    2. 2. 4 接种………………………………………………………………………………10
        2. 2. 4. 1 大麦的培育………………………………………………………………11
        2. 2. 4. 2 孢子悬浊液的制备………………………………………………………11
        2. 2. 4. 3 喷雾接种…………………………………………………………………11
    2. 2. 5 观察记载…………………………………………………………………………11
3 结果与分析……………………………………………………………………………………12
  3.1初筛……………………………………………………………………………………… 12
  3.2复筛……………………………………………………………………………………… 14
4 讨论……………………………………………………………………………………………15
  4.1 苗期接种发病情况影响因素分析……………………………………………………… 15
      4.1.1提高菌株的成活率…………………………………………………………………15
      4.1.2提高产孢量…………………………………………………………………………16
  4.2 下一步实验打算 …………………………………………………………………………16
文献 …………………………………………………………………………………………… 17
答谢………………………………………………………………………………………………18
附页………………………………………………………………………………………………19