转小粒野生稻基因种质野威B的产量与米质性状

转小粒野生稻基因种质野威B的产量与米质性状(9600字)
摘 要：野威B是将小粒野生稻（Oryza minuta）的基因组DNA通过穗茎注射法导入杂交水稻亲本V20B中培育出的转基因水稻新种质。与亲本V20B相比，野威B的有效穗数和每穗总粒数小于V20B，但每穗实粒数却高于V20B，其平均结实率比V20B高9 %，但其千粒重比V20B的少6 g。野威B倒1叶和倒2叶叶鞘中可溶性糖含量逐渐降低，其中第3个时期（乳熟期）降低的幅度最大，而V20B倒1叶和倒2叶叶鞘中可溶性糖含量降低幅度不如野威B明显。野威B的糙米率和整精米率与V20B的相近，但其垩白粒率要比V20B的低47.5%，垩白面积平均少15.7%。野威B的胶稠度比V20B的更高，而直链淀粉含量比V20B的低7.4%。
关 键 词：小粒野生稻；基因组DNA；野威B；产量；米质性状

Studies on the yield and quality characteristics of YeiweiB plants transformed with Oryza minuta genomic DNA
Abstract: Yewei B, a new rice germplasm was bred through transformed with genomic DNA of Oryza minuta into V20B by earstem injecting. Compared with V20B, the effective panicles numbers and the spikelets number per panicles of Yewei B are smaller than that of V20B, but the filled grains per spike is higher than that of V20B. The average seed setting percentage of Yewei B is found to be 9 % higher than that of V20B, but 1000-grain weight of Yewei B is 6 gram less than that of V20B. The content of soluble sugar in leaf sheath of the top1st and top 2nd leaf decreases with the stage gradually in Yewei B，and the decreased range of milk stage was found to be the biggest one. Compared with Yewei B, the decreased range of soluble sugar in leaf sheath of the top1st and top 2nd leaf in V20B is less distinct. The brown rice percentage and head rice rate of Yewei B is similar to V20B, but the percentage of chalky grain is 47.5% and the area of chalkiness is 15.7 % lower than that of V20B respectively. Gel consistency of Yewei B is higher and the content of amylose is low than that of V20B.
Key words: Oryza minuta; genomic DNA; Yewei B; yield, rice quailty
 

目    录 
1  前言    …………………………………………………………………………………………3
1.1  国内外研究进展    ……………………………………………………………………3
1.1.1  转野生稻种质的发展前景    …………………………………………………3
1.1.2  米质基本性状研究    …………………………………………………………4
1.1.3  项目检测方法    ………………………………………………………………5
1.1.4  本研究的目的    ………………………………………………………………8
1.1.5  本研究小粒野生稻的意义    …………………………………………………8
2  材料与方法    …………………………………………………………………………………9
2.1  材料    …………………………………………………………………………………9
2.2  试验设计…………………………………………………………………………9
2.3  项目测定及方法…………………………………………………………………9
3  结果与分析    …………………………………………………………………………………9
3.1  转Oryza minuta基因组DNA水稻野威B的产量性状    …………………………9
3.2  转Oryza minuta基因组DNA水稻    ………………………………………………9
3.3  转Oryza minuta基因组DNA水稻野威B次库中可溶性糖含量变化……………10
4  小结    ………………………………………………………………………………………11
5  讨论    ………………………………………………………………………………………12
参考文献    ……………………………………………………………………………………12
致  谢    ………………………………………………………………………………………14