防治柑橘溃疡病菌药剂的筛选

防治柑橘溃疡病菌药剂的筛选(7400字)
摘  要：筛选对柑橘溃疡病菌抑制作用的药剂是防止柑橘溃疡病菌的主要方法。液体振荡培育出纯种病菌，将菌液均匀涂抹在牛肉膏蛋白胨琼脂培养基上，中间用打孔器打上孔滴定选定药液，然后测量孔周围抑制圈的大小，来判断药液抑制效果。经实验结果显示，其中15%恶霉灵的抑菌效果最好，其EC50为7.45μg/mL；其次为植保素、25％溴菌腈可湿性粉剂，其EC50分别为16.26μg/mL和23.57μg/mL；20%松脂酸铜的效果最差，其EC50已近达到了111.71μg/mL。15%恶霉灵的抑菌效果最好，其EC50为7.45μg/mL。
关键词：柑橘溃疡病菌；恶霉灵；植保素；溴菌晴；松脂酸铜。

Selection of chemicals to prevent the ulcer germs in citrus
Abstract: The choice of the drugs inhibiting citrus canker germ is the major way to prevent it. Liquid oscillation cultivate purebred germs, and we can spread bacteria liquid equably on beef extract peptone agar medium, among which puncher print a hole to select liquid medicine, and then measure the size of inhibiting ring around the hole to judge the inhibition of liquid medicine.  Experimental result shows the inhibition of 15% hymexazol is the best, and EC50 is 7.45μg/mL. then phytoalexin  is the second best, and 25% bromothalonil is wetter, the EC50 16.26μg/mL and 23.57μg/mL respectively; The effect of 20%copper abietate is the worst, and EC50 has nearly reached 111.71μg/mL L. The inhibition of 15% hymexazol is the best, and EC50 is 7.45μg/mL.
Key words: citrus canker bacteria；hymexazol；phytoalexin；bromothalonil；copper abietate.

目    录
摘  要    1
关键词    1
1 前言    2
1.1 柑橘溃疡病境外分布    2
1.2 柑橘溃疡病菌寄主范围    2
1.3 柑橘溃疡病的危害情况    2
1.4 柑橘溃疡病症状    2
1.4.1 柑橘溃疡病病叶症状    2
1.4.2 柑橘溃疡病枝梢症状    3
1.4.3 柑橘溃疡病果实症状    3
1.4.4 柑橘溃疡病原菌形态    3
1.4.5 柑橘溃疡病传播途径    3
1.5 柑橘溃疡病国内现状    3
2 材料和方法    4
2.1 供试材料    4
2.2 固体和液体培养基的制作与灭菌    4
2.2.1 固体牛肉膏蛋白胨琼脂培养基    4
2.2.2 牛肉膏蛋白胨液体培养基的制作    5
2.3 柑橘溃疡病菌的分离与纯化    5
2．4 柑橘溃疡病菌的培养    6
3 柑橘溃疡病化学药剂的筛选方法    6
3.1 化学药品的稀释    6
3.3 各化学药剂对柑橘溃疡病病原菌抑制作用的测定    7
4 结果与分析    7
4.1 不同药剂对柑橘溃疡病病原菌生长的影响    7
4.2 药剂浓度对柑橘溃疡病病原菌抑制作用    7
4.3 不同药剂对柑橘溃疡病病原菌的室内毒力比较    8
5小结与讨论    9
参考文献    10
致    谢    11